The Molecular Biotechnology Center has started to be active in the new location in Via Nizza 52, with the first groups in September 2006. It is expected that by March 2007 all the Groups will be active in the new building. With the availability of the new premises new research lines have been activated. Next to the use of the mouse model for embryonic development and gene regulation, a “state of art” zebrafish facility is now operative. Within the subject of stem cell differentiation, thanks to the support from Telethon Foundation, we have recruited an investigator with experience in epithelial stem cells. Thanks to the support from SanPaolo Foundation, that has been and continues to be essential, we have been able to equip the Center with those modern instruments needed for sophisticated experiments. Within the field of molecular imaging, in addition to magnetic resonance acquired by the Center of Excellence, we have added a sophisticated apparatus for optical imaging that permits to visualize differentiation both in the mouse and the zebrafish models, at the cellular level.